Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Effect of different concentrations of Ang II on migration of human bone marrow MSCs. a Nondirectional migration ability of human bone marrow MSCs after stimulations with different concentrations of Ang II (10 –8 , 10 –7 , 10 –6 , 10 –5 , and 3 × 10 –5 M) examined using the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of human bone marrow MSCs after stimulations with the different concentrations of Ang II indicated examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. n = 3; * p < 0.05 vs normal. Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy

Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Effect of AT1R and AT2R antagonists on Ang II-mediated migration of MSCs. a Nondirectional migration ability of MSCs after stimulation with 100 nM Ang II following pretreatment with Losartan (5 μM) and/or PD-123319 (5 μM) examined with the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of MSCs after stimulation with 100 nM Ang II following pretreatment with Losartan (5 μM) and/or PD-123319 (5 μM) examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. Significant differences were found compared with the normal group ( n = 3; * p < 0.05) and compared with the control group ( n = 3; # p < 0.05). Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy, Control

Journal: Stem Cell Research & Therapy

Article Title: Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro

doi: 10.1186/s13287-017-0617-z

Figure Lengend Snippet: Roles of FAK and Rho GTPases in the migration of MSCs. a Nondirectional migration ability of MSCs after stimulation with Ang II and/or inhibitors of FAK and the Rho GTPases examined using the scratch assay. Wound sites (areas cleared of cells in the center of the scratched area) were observed and photographed at 0 and 24 h (200×). b Quantitative results of wound healing. c Directional migration ability of MSCs after stimulation with Ang II and/or inhibitors of FAK and the Rho GTPases examined using the Transwell migration assay. Migrated cells on the bottom surfaces of the Transwell inserts were stained with crystal violet and observed under a microscope (200×). d Quantitative results of cell migration. n = 3; * p < 0.05 vs normal, # p < 0.05 vs the control group. Ang II angiotensin II

Article Snippet: Modified Boyden chamber assays were conducted using Transwell polyester membrane filter inserts with 8-μm pores (Corning Inc., Corning, NY, USA) at a density of 500,000 cells/ml per Transwell (upper chamber) as described previously [ ].

Techniques: Migration, Wound Healing Assay, Transwell Migration Assay, Staining, Microscopy, Control

Journal: iScience

Article Title: The SW480 cell line as a model of resident and migrating colon cancer stem cells

doi: 10.1016/j.isci.2024.110658

Figure Lengend Snippet:

Article Snippet: For the transwell (modified Boyden chamber) migration and assay, 5.0 × 10 4 cells were re-suspended in DMEM medium and plated in transwell tissue culture inserts (8 μm polycarbonate membrane, 24 well, 3428; Corning).

Techniques: Recombinant, Reporter Assay, SYBR Green Assay, Plasmid Preparation, Software